Seven different alleged victims were mentioned among the 22 charges.
The girls ranged in age from 9 to 29 at the time.
According to the lawsuit, Nassar would give the Olympians "pain treatments" where he would allegedly insert his ungloved hands into their vaginas.
Schuette said more charges were coming.
Schuette and Michigan State University Police Chief James Dunlap are holding a news conference at 2:30 p.m.in Lansing. The Karolyis deny any role in or knowledge of abuse and said they were unaware of complaints against Nassar until recently.
"This guy is a monster", Schuette said. "I encourage anyone who may have been a victim of Larry Nassar to come forward by contacting the MSU Police Department".
Michigan Attorney General Bill Schuette is expected to provide an update Wednesday morning on the investigation into former Michigan State University gymnastics doctor Larry Nassar. Schuette called that claim "outrageous". Messages seeking comment were left with his lawyers.
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Nassar, jailed in MI on child pornography and criminal sexual conduct charges, has denied the allegations and says his treatments were legitimate medical practices. The charges were the first that included crimes committed while serving in his role as a team physician at Michigan State.
Details were disclosed in affidavits presented Wednesday by a Michigan State University police detective.
He was sacked by Michigan State last September for failing to follow restrictions that were put in place after a 2014 complaint about his treatments. "But the story here is that no one was watching to protect these girls, and they (USA Gymnastics and the Karolyis) put medals and money first". Dr. Nassar preyed on these young girls, just little girls.
"The allegations of sexual assault against Dr. Nassar continue to increase almost every day, and we remain constantly in contact with the victims as we move forward", Michigan State University Police Chief Jim Dunlap said in the press release. She says the girls trusted Nassar and saw him as a "god to the gymnasts".
Antolin said she only realized previous year what she thought was treatment had been abuse.
In November, Nassar was charged with three counts of first-degree criminal sexual conduct in the abuse of a child under the age of 13 in his home in Holt from 1998-2005. Those cases are pending. MSU began investigating Nassar in August, a month before an explosive report by the Indianapolis Star over USA Gymnastics' handling of sexual abuse allegations.
"He would put his fingers inside of me, move my leg around", Dantzscher told CBS' "60 Minutes" in an interview broadcast Sunday. He would tell me I was going to feel a pop.